Effect of Grape Seed Extract on the T-2 Toxicity in mice

Abstract

T-2 toxin is a cytotoxic secondary fungal metabolite that belongs to the trichothecenes mycotoxin family. Hepatotoxicity and nephrotoxicity were induced in mice by feeding T-2 toxin contaminated wheat. The serum biochemical analysis was observed after 8 weeks by an increase in the plasma activity of alanine aminotransferase (ALT), where that elevation reached (76.00 IU/L) in G2, in comparison with G3 that the ALT level return back to normal level. While the levels of total protein and albumin appeared no significant change in all groups of study. Serum urea showed a significant increase in the mean serum level of urea (52.70 mg/dl) in G2 whereas the treated with grape seed extract could return back the mean serum level of urea to normal level (38.95 mg/dl). Serum level of total bilirubin was observed increase significantly to reach (0.42 mg/ dl) in G2 when compared to third group that the grape seed extract reduce the serum level of total bilirubin to normal level (0.34 mg/dl). There was a significant increase in the mean serum level of creatinine was observed in the T-2 toxin group (0.69 mg/dl) when compared to creatinine level in grape seed extract with T-2 toxin recorded a significant reduction (0.54 mg/dl). Histopathological examination of liver and kidney sections confirmed the serum analysis. These results clearly indicated that T-2 toxin has stressful effects on the hepatic and renal tissues.