Correlation Between Tumor Suppressor Gene P53 And Bladder Cancer In Some Iraqi Patients
Alterations of tumor suppressor gene p53 were identified in a various types of human cancers, including bladder cancer . So , the present study was designed to investigate the relationship between the tumor suppressor gene p53 in some Iraqi patients with bladder cancer by using two methods , insitu hybridization compare with immunohistochemical assay. A series of 40 bladder tissue cancer collected from different sites (transitional cell and squamous cell) from patients who had undergone cystactomy. These samples were collected during the period between (2012-2014). The histologic types included 21 well differentiated transitional cell , squamous cell, 9 moderately differentiated and ten poorly differentiated carcinomas. By using in situ hybridization , tumor suppressor gene p53 was detected in eighteen out of fourty (45%),otherwise, at immunohistochemistry , it was detected in twenty eight out of fourty (70%). Moreover, the positive results of tumor suppressor gene p53 was related in highly significant to each of stages and sites , significantly related with histologic grade of the tumor by using insitu hybridization method , but there was no significant correlation with each of age and smoking patients. furthermore , in immunohistochemical assay p53 related in highly significant correlation with each of histologic grade, sites, stages of the tumor and the smoking habit. So, it was significantly related with age and gender. It could be conclouded from this study that the two methods of in situ hybridization and immunohistochemistry for detection of tumor suppressor gene , protein p53 is useful in the clinical evaluation of patients with bladder cancer and it suggested that mutations of p53 are involved in the bladder transitional cell and squamous cell carcinomas and it may play an a major role in the malignant transformation of urinary tract cancer.