Growth inhibition of mice mammary carcinoma cell line with green synthesized Magnetic Iron Oxide Nanoparticles
Mammary adenocarcinoma represents one of the most cancers that effect woman in Iraq and worldwide. Finding a definite
cure for this type of cancer is a target of considerable research in many laboratories over the globe. In this study, a green
synthesized magnetic iron oxide nanoparticles (MNP) was used to specify their ability to inhibit the growth of mice mammary
carcinoma cell line (AMN3), it comes as a preliminary study of wider ongoing research. After successfully synthesizing magnetic
iron nanoparticles, it employed in different concentrations to expose AMN3 cells for 24 and 48 hours. The viability of the
exposed cells was determined using standard MTT assay. The inhibition concentration 50 was determined afterward and used
to test the possibility of inducing apoptosis in the carcinoma cells using a mixture of fluorescent stains acridine orange and
propidium iodide. Results indicated that green synthesized MNP can inhibit the growth of mammary adenocarcinoma cell line
up to 79% and 89% after 24 and 48 hr of incubation with 1000μg/ml. The IC50 of MNP was capable of inducing apoptosis in
this cell line as shown by fluorescence microscope. The standard hemolysis assay used to test the reaction between the green
synthesized magnetic oxide nanoparticles and normal cells. The IC50 of AMN3 cells growth inhibition only induce 14% of
RBC hemolysis, while the percentage induced by negative control was 10%. In conclusion, green MNP have an effect upon
cancer cells greater than normal cells. This material must tested against other types of cancer and normal cells to verify its
action and study mechanisms of apoptosis induction in detail.
Key words: Magnetic iron oxide nanoparticles, AMN3, green synthesis, nanotherapy.