P53 and GSTs Polymorphisms among Bladder Cancer Patients in Iraq

Abstract

The role of metabolic genes for glutathione S-transferase (gstm1, gstt1) and P53 exon-7 and 8 as risk factors for bladder cancer in Iraqi patients was studied. Thirty five samples from patients attended the Nuclear Medicine Hospital in Baghdad plus fifty samples from apparently healthy individuals were included in the study. DNA extraction was performed using proteinase K/ SDS method PCR techniques were followed to amplify the gene using specific primers for detection of gstm1, gstt1 and albumin as internal control. Single strand conformation polymorphism (PCR-SSCP techniques) for detection of point mutation in P53 exon-7 and 8 were used. Genetic analysis showed that gstm1 null genotype was statistically associated with bladder cancer (14.29%), this association was strengthened when both gstm1 and gstt1 alleles were absent (28.5%). For detection of the point mutations in P53 exon-7 and 8 Single Strand Conformation Polymorphism (PCR-SSCP ) have revealed that there was high association between exon-7 mutations and incidence of bladder cancer (42.86%), while the association between exon-8 mutations and bladder cancer was low (14.28%). In case of combination between exon-7 and 8 mutations, the association was also low (11.42%). Mutations in gstm1 and exon-7 being associated together are more than with exon-8 mutations. The number of mutations in P53 gene was more in patients with combined genotype of gstm1 deletion and null genotypes