Inhibition the Growth of Some Malignant Cells Lines by Alfalfa Aqueous Extract

  • Rafid abdul wahid. A.

Abstract

The aim of the present study was to assess the cytotoxic activity of aqueous extracts of Alfalfa (Medicago sativa ) by using (MTT) (colorimetric assay) to measure the cytotoxic activity on two malignant tumor cell lines (AMN3 ) Ahmed Mohamed Nahi 2003 and (GB) Glioblastoma cell line ,and one type of normal cell line (MEF) Mouse Embryo Fibroblast cell line, through different exposure time (24, 48 ,and 72hrs ) and different concentration of aqueous extract ; (1000,500,250,125 ,and 62.5, mg/ml), the study was carried out in the Iraqi Center for Cancer and Medical Genetics Research ,The result showed that the aqueous extracts from alfalfa have significant effects P value ≤ 0.05 on the growth of AMN3 cell lines and GB malignant cell line in culture in dose and time- dependant manner ,Viability of cell lines decreased with time and concentration reaching its lowest value after 72 hrs of treatment with the highest concentration used (1000 mg/ml ). The results also indicated that GB cell lines were more sensitive to the Alfalfa aqueous extracts as compared with the growth of AMN3 cell lines . From the other side the result showed that there is no significant effect P value ≥ 0.05 of these aqueous extracts on the viability of normal cell line (MEF3 cell line). Only the treatment with higher concentration (1000µg/ml) after 72 hrs caused significant reduction P value ≤ 0.05 in the (MEF) cell line growth

Published
2018-01-12
How to Cite
A., Rafid abdul wahid.. Inhibition the Growth of Some Malignant Cells Lines by Alfalfa Aqueous Extract. Iraqi Journal of Cancer and Medical Genetics, [S.l.], v. 3, n. 2, jan. 2018. ISSN 2078-6123. Available at: <http://ijcmg.uomustansiriyah.edu.iq/index.php/ijcmg/article/view/45>. Date accessed: 11 aug. 2020.
Section
Articles

Most read articles by the same author(s)

Obs.: This plugin requires at least one statistics/report plugin to be enabled. If your statistics plugins provide more than one metric then please also select a main metric on the admin's site settings page and/or on the journal manager's settings pages.