Screening for Genetic Polymorphisms of Glutathione- S-Transferase Genes and Risk Factors Among Breast Cancer Patients in Iraq

Abstract

The study included 40 Iraqi women diagnosed as breast cancer patients who attended the Nuclear Medicine Hospital in Baghdad and 60 apparently healthy women as control .Genomic DNA extraction was performed using proteinase K / SDS method. Multiplex PCR techniques were followed to amplify the genes using specific primers. for detection of gstm1, gstt1 and albumin gene as a control. Genetic analysis showed that the percentage of deletions in breast cancer patients was 75% (gstm1 27.5%, null genotype 32.5 % and 15% for gstt1) versus 10 % were in the control samples (5 % gstt1, 3.33% gstm1 and 1.67% null genotype). Data were subjected to statistical analysis using Chi square ( X2 test ) to evaluate the association between etiological risk factors and having a risk for breast cancer . A significant association was considered at level ( P < 0.05 ) . Blood group O and A showed an interesting association with breast cancer risk. The percentage of deletion was 90.9% and 86.6% in O and A blood group, respectively. Age and family history had no significant risk association with breast cancer (P > 0.05) in this study