Potential Cytotoxic Effect of Crude Extract Produced by Lactobacillus spp on Caco-2 & L20B Cell Lines in vitro
This study was designed to determine the cytotoxic effect of crude products which was produced by isolates of Lactobacillus
spp against three cell lines using 3-(dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT assay). About 72
samples were collected from local dairy products and infant feces with age of (1- 40 days) in Baghdad, the collection of those
samples last long (September 2016 to January 2017). All isolates were identified as Lactobacillus spp according to their morphological,
cultural, biochemical features and PCR technique by 16SrRNA gene. The cytotoxicity of crude extract was evaluated
on cancer intestinal cells of mice L20B, human colon adenocarcinoma Caco-2 and human hepatic WRL-68 cell lines. The
results showed that crude extract had cytotoxic effect on L20B with IC50= 17 g/ml and on Caco-2 with IC50=221.9 g/
ml in concentration 400 g/ml for both. While the crude extract of WRL-68 cells did not show when treated with the same
concentration when used WRL-68 as normal cells for comparison. Conclusion: In last years, the high prevalence rate of many
types of cancer in Baghdad, especially of intestinal cancer in human, so had used materials was produced by Probiotic bacteria
to study their effects on the cancer cells in vitro.
Key words: MTT assay, Caco-2 cell line, L20B cell line, Crude extracts, Lactobacillus spp.