Study of cytotoxicity of purified toxin A produced from pseudomonas aeruginosa on cell lines

Abstract

Pseudomonas aeruginosa is the leading etiology of gram negative bacteria causing diseases at most medical centers . The bacterium’s virulence depends on number of cell-associated and extracellular factors. Exotoxin A among these virulence factors are toxic for a wide variety of cell line, it inhibits protein synthesis in these lines as well as in numerous organs in animals models. In vitro cytotoxic effects of purified toxin A on two cell lines Rat embryo fibroblast cell line (REF) and on tumor cell line Rhabdomyosarcoma cell line (RD) were studied. Results revealed that toxin A had significant cytotoxic effects on both cell lines. Conclusion: In vitro evaluating the cytotoxic effects of purified toxin A produced from pseudomonas aeruginosa at different concentration on normal cell line Rat embryo fibroblast cell line (REF) ) and on tumor cell line Rhabdomyosarcoma cell line (RD) revealed that purified exotoxin A has significant inhibitory effects on REF cell line which was dose and time dependent. On the other hand cytotoxic effects of purified toxin A on tumor cell line (RD) reveal that inhibition in cell viability was dependent on concentration of purified toxin A but independent on time. Key words : Toxin A, cytotoxicity assay, cell line