Study The Effect of Purifed Goat Milk Lactoferrin on HeLa Cancer Cell line Growth In vitro

Abstract

Lactoferrin is an important protein in many biological applications as a potential cancer treatment agent .In this study, lactoferrin was purifed from goat colostrum by Ion exchange chromatography by using CM-Sephadex G-150 column and gelfltration by using Sephadex G-200 column .To evaluted its ability as anticancer agent the study utilized an in vitro evaluation for the cytotoxic effect of the purifed goat milk lactoferrin (gLf) on two cell lines, HeLa (Human cervical cancer) cell line and Rat Embryo Fibroblast (REF) cell line at different concentrations and different exposure time of treatment. The purifed gLf concentrations ranging (19.53 to 5000) μg/ml for 24,48 and 72 hours. The effect of gLf was evaluated by employing MTT assay. The results revealed signifcant cytotoxic effect at levels (P<0.05) for all concentrations and for all exposure time of gLF on HeLa cell line as compared to untreated control cells, The inhibition rate IR% increased with raising of gLF concentration and incubation period The highest inhibitory growth was at the concentration ( 5000 µg/ml) after 72hrs of exposure time (64.38%) ,while only the highest concentration gave signifcant inhibitory effect (P< 0.05) with normal cell REF .