The Cytotoxic Activity of Punica granatum on Growth of Hela and REF Cell Lines

Abstract

The crude aqeouse extracts of punica granatum peel were assessed for their cytotoxic activity assay.
This study utilized two different types of cell lines, Hela (Cervical human carcinoma) and REF (Rat Embryo fibroblast).
The results showed that the aqueous extract of punica granatum peel exhibited high cytotoxicity against the REF cell line with an inhibition value of 97.3 μg/ml after 24 h incubation. However, punica granatum peel displayed less toxicity against the Hela cell line with an inhibition value 24.4 μg/ml after 24 h incubation and this effect was dose- and time-dependent.
In conclusion, the results demonstrated that punica granatum peel is potently cytotoxic against REF cell, and lesser extend against Hela cells.