Molecular characterization of some resistance genes of E.coli isolated from patients with prostate cancer

Characterization of prostate cancer bacteria

Abstract

The current study was conducted to identify pathogenic E.coli that coexist with prostate cancer and characterize these bacteria based on some virulence genes using molecular techniques such as RAPD-PCR. The study was carried out on 200 samples isolated from prostate cancer patients in different hospitals in Maysan Province, Iraq. These samples were cultivated on traditional and specific media agars for the identification of certain pathogenic bacteria. These bacterial organisms were subjected to PCR targeting the 16S rRNA gene for the identification of these bacteria and the genes aac2, blaoxA-1, blaTEM, oxA48 for the detection of virulence factors of isolated bacteria. The results revealed the presence of 200 bacterial isolates, which included 65 (59.09%) E. coli, 20 (18.18%) Proteus mirabilis, 12 (10.9%) P. penneri, 10 (9.09%) Pseudomonas aeruginosa, and 3 (2.72%) Klebsiella pneumonia, 16 (32%) Enterococcus faecalis, 15 (30%) Staphylococcus hemolyticus, 9 (18%) S. lentus, 3 (6%) Aerococcus viridans, , and 7 (14%) Streptococcus uberis. The findings of 16S rRNA-PCR confirmed the results of conventional tools. The virulence genes were detected in the identified bacterial species. The study findings reveal a diverse community of bacterial species present in samples isolated from patients with prostate cancer with high virulence, detected by the presence of virulence genes.