Inhibition the Growth of Some Malignant Cells Lines by Alfalfa Aqueous Extract

Abstract

The aim of the present study was to assess the cytotoxic activity of aqueous extracts of Alfalfa (Medicago sativa ) by using (MTT) (colorimetric assay) to measure the cytotoxic activity on two malignant tumor cell lines (AMN3 ) Ahmed Mohamed Nahi 2003 and (GB) Glioblastoma cell line ,and one type of normal cell line (MEF) Mouse Embryo Fibroblast cell line, through different exposure time (24, 48 ,and 72hrs ) and different concentration of aqueous extract ; (1000,500,250,125 ,and 62.5, mg/ml), the study was carried out in the Iraqi Center for Cancer and Medical Genetics Research ,The result showed that the aqueous extracts from alfalfa have significant effects P value ≤ 0.05 on the growth of AMN3 cell lines and GB malignant cell line in culture in dose and time- dependant manner ,Viability of cell lines decreased with time and concentration reaching its lowest value after 72 hrs of treatment with the highest concentration used (1000 mg/ml ). The results also indicated that GB cell lines were more sensitive to the Alfalfa aqueous extracts as compared with the growth of AMN3 cell lines . From the other side the result showed that there is no significant effect P value ≥ 0.05 of these aqueous extracts on the viability of normal cell line (MEF3 cell line). Only the treatment with higher concentration (1000µg/ml) after 72 hrs caused significant reduction P value ≤ 0.05 in the (MEF) cell line growth