Using Silver Nano- Particles to Increase Efficiency Of Sterile Solution for in vitro Techniques

Abstract

This work was done by using explants cultured in MS medium supplemented with 3mg/l of NAA plus BAP, 30g/l sucrose beside different concentrations of silver nano particles 4,8,12 mg/l. to assess the efficiency of using these particles in control the infection percent. The results indicate that healthy plants percent were increase from 45% to 100% in all treatments. However, in 4 mg/l treatment the explants were continued to grow better even after 28 days in culture. This is the first report on using silver particles in vitro technique especially in Iraq .