Comparison between Spinacia Oleracea and FeSO4 as a Source of Iron to Treated Anemia in Mice
I n this study thirty healthy male mice (Swiss albino aged of eight week) were used and divided in five groups. Three groups were feed with bread made of flour containing spinach (three different percentage 10%, 20%, and 30% respectively) as source of iron, compare with group number four which was feed with bread made of flour fortified with premix (consist of FeSO4, Folic acid and Maize starch). It is add according to the information given by the supplier (200g/ 1000 kg flour). Group number five was the control (feed with bread made of unfortified flour). Same food was used during the time of the experiment (fourteen days). The study was designed to make anemia in all groups by injection with phenylhydrazine . Samples of blood were taken by bleeding through heart puncture to measure the hemoglobin (Hb) and the packed cell volume (PCV). At the end of the experiment, mice in all the groups were then sacrificed and slides from the tissues of heart, kidney, liver and spleen had been made to measure the histological effects of these sources of iron. Results showed that there were significant differences in weights, (Hb) and (PCV) between mice feed with bread containing (10%, 20% and 30%) spinach and that containing premix and the control. In the same time slides of harts, kidneys, livers and spleens did not showed any detectable effect.