Expression of MAO gene in both starved and non-starved glioblastoma cancer cell line
Expression of MAO gene in glioblastoma
DOI:
https://doi.org/10.29409/ijcmg.v16i1.256Keywords:
Iraqi cancer registry, serum starvation, glioblastoma cancer cell lines, MAO gene expressionAbstract
Background: The relationship between cancer cell aggression induced by serum starvation and cell metastasis has been studied. Serum starvation is a typical way to induce tumor cell apoptosis, and stress may affect cell growth. Monoamine oxidase A (MAOA) was discovered as a mitochondrial enzyme that plays a role both as tumor suppressor and as an oncogenic factor in promoting tumor cell growth. Glioblastoma is a deadly cancer in the 4th rank reported in the Iraqi Cancer Registry 2020.
Aim of the study: The deprivation of serum from cultured media for glioblastoma cancer cell line (AMGM5) was tested after three different durations 24, 48, and 72h.
Methods: Comparison of MAO gene expression between starved and non-starved glioblastoma cell lines was measured using real-time PCR.
Results: The results indicated an increase in MAO gene expression in serum deprivation of cultured cells compared significantly (p<0.03) to non-starved cancer cell lines.
Conclusion: the increase in MAO expression in well-established cell populations of the AMGM5 cancer cell line in a serum-containing microenvironment compared to a lower MAO level in starved cells suggests the importance of cancer therapy using deprived microenvironments.
Downloads
Published
Issue
Section
License
Copyright (c) 2023 Iraqi Journal of Cancer and Medical Genetics

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.