Study of the Anticancer Activity of Annona squamosa Seeds
Anticancer Activity for Annona seeds
DOI:
https://doi.org/10.29409/xqq6c722Keywords:
Annona seeds, Anticancer activity, MCF-7 cell line, MDA cell line, REF cellsAbstract
The whole world is still searching for natural sources in various fields, especially natural alternatives for treating diseases and various health disorders, most notably in the treatment of tumors and cancers. The current study included an in vitro anticancer estimation of Annona seed extract on two cell lines of breast cancer (MCF-7& MDA-231) and one normal cell line represented by REF. Active constituents in the seeds were extracted by maceration with 70% ethanol. General phytochemical tests were conducted to investigate the active constituents of the extracted seeds. The phenolic compounds and flavonoids in the extracted residue were estimated via HPLC in addition to the nonpolar substances in the seed residue. The three cell lines (MCF-7, MDA-231 & REF) were subjected to different concentrations of seed extract for the anticancer assay. Results showed that the ethanolic extract residue was 8.4 g from 90 g of powdered plant seeds, representing 9.34% W/W, and the seed residue contained many active constituents. The HPLC results indicated that the seed extract was rich in gallic acid, caffeic acid, pyrogaloll, cinnamic acid, p-coumaric acid and, to a lesser extent, chlorogenic acid, which are simple phenolic compounds. Luteolin, kaempferol, and apegnine and lower amounts of quercetin, rutin and catechin, which are flavonoids, were also detected in the residue extracted from the seeds. For the nonpolar seeds, the following components were detected at a decreasing level: β-myrcene, p-cymene, α-pinene, eugenol, and β-pinene. The anticancer activity of the seed extract at several concentrations differed across the three types of cell lines in the present study.
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