Antiproliferative Activity of Spironolactone: In Vitro Study

Abstract

Spironolactone SPL stock solution was studied against two cancer cell lines Hep-2, and AMN-3. These cell lines were exposed to different concentrations of SPL ranged from 0.0019 to 1000 µg/ml for 24, 48 and 72 hours durations. The optical density was measured under wavelength 492 nm with micro-ELISA reader after their staining with crystal violet dye. The results revealed a clear cytotoxic activity of pure SPL with high significances in a two cancer cell lines during the three exposure time, suggesting that the cytotoxic effect of pure spironolactone is a dose and time dependant. The best cytotoxic activity was on AMN-3 and Hep-2 cell lines at the high concentrations 1000, 500, 250, 125µg/ml. The results also suggest that AMN-3 cell line is the most sensitive cancer cell line to pure SPL than Hep-2 cell line after 24 hr. of exposure to drug